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IFT122 Polyclonal antibody

IFT122 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19304-1-AP

Synonyms

WDR10p, WDR10, WD repeat-containing protein 140, WD repeat-containing protein 10, SPG

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using IFT122 Polyclonal antibody (19304-1-AP)

    WB analysis of mouse testis using 19304-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19304-1-AP (IFT122 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human testis using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human testis using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human brain using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human brain using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human stomach using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human stomach using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using IFT122 Polyclonal antibody (19304-1-AP)

    IF Staining of MDCK using 19304-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT122 Polyclonal antibody (19304-1-AP)

    IF Staining of hTERT-RPE1 using 19304-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT122 Polyclonal antibody (19304-1-AP)

    IF Staining of hTERT-RPE1 using 19304-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19304-1-AP targets IFT122 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT122 fusion protein Ag6860
Full Name intraflagellar transport 122 homolog (Chlamydomonas)
Calculated Molecular Weight 142 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC004238
Gene Symbol IFT122
Gene ID (NCBI) 55764
RRIDAB_2714184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IFT122 antibody 19304-1-APDownload protocol
IHC protocol for IFT122 antibody 19304-1-APDownload protocol
IF protocol for IFT122 antibody 19304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua
View Article
mouseWB,IF

Elife

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

Authors - Tooba Quidwai
View Article
humanWB

Hum Mol Genet

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.

Authors - Mariko Takahara
View Article