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  • Featured Product
  • KD/KO Validated

CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18832-1-AP

Synonyms

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using CEP192 Polyclonal antibody (18832-1-AP)

    IP experiment of HeLa cells using 18832-1-AP

    IP result of anti-CEP192 (18832-1-AP for IP and Detection) with HeLa cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CEP192 Polyclonal antibody (18832-1-AP)

    IHC staining of human liver cancer using 18832-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18832-1-AP (CEP192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CEP192 Polyclonal antibody (18832-1-AP)

    IHC staining of human liver cancer using 18832-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18832-1-AP (CEP192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP192 Polyclonal antibody (18832-1-AP)

    IF Staining of hTERT-RPE1 using 18832-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP192 antibody (18832-1-AP) at dilution of 1:150 and Cy3-conjugated Goat Anti-Rabbit IgG(H+L), SCLT1 antibody (14875-1-AP, Magenta), CoraLite®488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP192 Polyclonal antibody (18832-1-AP)

    IF Staining of HeLa using 18832-1-AP

    Immunofluorescent analysis of HeLa cells using 18832-1-AP (CEP192 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP192 Polyclonal antibody (18832-1-AP)

    IF Staining of HeLa using 18832-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP192 antibody (18832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP192 Polyclonal antibody (18832-1-AP)

    IF Staining of HeLa using 18832-1-AP

    Immunofluorescent analysis of HeLa cells using 18832-1-AP (CEP192 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18832-1-AP targets CEP192 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name centrosomal protein 192kDa
Calculated Molecular Weight 213 kDa
Observed Molecular Weight 250-300 kDa
GenBank Accession NumberBC144481
Gene Symbol CEP192
Gene ID (NCBI) 55125
RRIDAB_2878560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

Protocols

Product Specific Protocols
IHC protocol for CEP192 antibody 18832-1-APDownload protocol
IF protocol for CEP192 antibody 18832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Mechanism of spindle pole organization and instability in human oocytes.

Authors - Chun So
View Article
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
View Article
mouseIF

EMBO Rep

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Authors - Ting Song
View Article
humanWB,IF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
View Article
mouseIF

PLoS Genet

Aurora kinase A is essential for meiosis in mouse oocytes.

Authors - Cecilia S Blengini
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article