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ADAMTSL4 Polyclonal antibody

ADAMTSL4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15304-1-AP

Synonyms

ADAMTS like 4, ADAMTS like protein 4, ADAMTSL 4, ADAMTSL4, TSRC1, UNQ2803/PRO34012

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    WB analysis of mouse brain using 15304-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    WB analysis of human liver using 15304-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    WB analysis of mouse lung using 15304-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    WB analysis of mouse skeletal muscle using 15304-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IP experiment of mouse brain using 15304-1-AP

    IP result of anti-ADAMTSL4 (IP:15304-1-AP, 4ug; Detection:15304-1-AP 1:500) with mouse brain tissue lysate 3440ug.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of mouse skeletal muscle using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of mouse skeletal muscle using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of human liver using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of human skeletal muscle using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of human skeletal muscle using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of human liver using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of human colon cancer using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADAMTSL4 Polyclonal antibody (15304-1-AP)

    IHC staining of human colon cancer using 15304-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human colon cancer tissue, human liver tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15304-1-AP targets ADAMTSL4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAMTSL4 fusion protein Ag7781
Full Name ADAMTS-like 4
Calculated Molecular Weight 117 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC027478
Gene Symbol ADAMTSL4
Gene ID (NCBI) 54507
RRIDAB_2222624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADAMTSL4 antibody 15304-1-APDownload protocol
IHC protocol for ADAMTSL4 antibody 15304-1-APDownload protocol
IP protocol for ADAMTSL4 antibody 15304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,IF

Matrix Biol

Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.

Authors - Rohtem Aviram
View Article
humanIHC, WB

Front Mol Biosci

An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.

Authors - Hongpan Zhang
View Article
humanWB

Oncotarget

Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.

Authors - Ya Tao
View Article
ratIHC

Int J Nanomedicine

Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment

Authors - Junyan Chen
View Article