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NEDD1 Polyclonal antibody

NEDD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13993-1-AP

Synonyms

GCP WD, NEDD 1, NEDD1, Protein NEDD1, TUBGCP7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis using 13993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis of human brain using 13993-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using NEDD1 Polyclonal antibody (13993-1-AP)

    WB analysis of SH-SY5Y using 13993-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NEDD1 Polyclonal antibody (13993-1-AP)

    IP experiment of HeLa using 13993-1-AP

    IP result of anti-NEDD1 (IP:13993-1-AP, 3ug; Detection:13993-1-AP 1:800) with HeLa cells lysate 2150ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human liver using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human liver using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human colon using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NEDD1 Polyclonal antibody (13993-1-AP)

    IHC staining of human colon using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13993-1-AP targets NEDD1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD1 fusion protein Ag5077
Full Name neural precursor cell expressed, developmentally down-regulated 1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 75 kDa, 62 kDa
GenBank Accession NumberBC027605
Gene Symbol NEDD1
Gene ID (NCBI) 121441
RRIDAB_2149202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.

Protocols

Product Specific Protocols
WB protocol for NEDD1 antibody 13993-1-APDownload protocol
IHC protocol for NEDD1 antibody 13993-1-APDownload protocol
IP protocol for NEDD1 antibody 13993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article