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  • Featured Product
  • KD/KO Validated

PPP2CA Polyclonal antibody

PPP2CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13482-1-AP

Synonyms

Replication protein C, PP2CA, PP2Ac, PP2A-alpha, PP2A alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis using 13482-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of Jurkat using 13482-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of MCF-7 using 13482-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of HEK-293T using 13482-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of human liver using 13482-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PPP2CA Polyclonal antibody (13482-1-AP)

    IP experiment of Jurkat using 13482-1-AP

    IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    IHC staining of human liver using 13482-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    IHC staining of human liver using 13482-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    IF Staining of MCF-7 using 13482-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    FC experiment of MCF-7 using 13482-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHEK-293T cells, human liver tissue, Jurkat cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13482-1-AP targets PPP2CA in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated Molecular Weight 309 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2169485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protocols

Product Specific Protocols
WB protocol for PPP2CA antibody 13482-1-APDownload protocol
IHC protocol for PPP2CA antibody 13482-1-APDownload protocol
IF protocol for PPP2CA antibody 13482-1-APDownload protocol
IP protocol for PPP2CA antibody 13482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.

Authors - Hai Zheng
View Article
mouseWB

Protein Cell

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

Authors - He-Zhou Huang
View Article
humanWB,IP

Cell Res

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Authors - Xiang Xu
  • KD Validated
View Article
humanWB

Acta Pharm Sin B

WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.

Authors - Xiaomeng Gao
View Article
mouseWB

Cardiovasc Res

Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.

Authors - Sarah-Lena Puhl
View Article
humanWB

EMBO J

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network

Authors - Cuirong Guan
View Article