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NIFK Polyclonal antibody

NIFK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12615-1-AP

Synonyms

MKI67IP, Nucleolar protein interacting with the FHA domain of pKI-67, NOPP34, MKI67 FHA domain-interacting nucleolar phosphoprotein, hNIFK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIFK Polyclonal antibody (12615-1-AP)

    WB analysis using 12615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12615-1-AP (NIFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NIFK Polyclonal antibody (12615-1-AP)

    WB analysis of HEK-293 using 12615-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12615-1-AP (MKI67IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human liver cancer using 12615-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human liver cancer using 12615-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human renal cell carcinoma using 12615-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NIFK Polyclonal antibody (12615-1-AP)

    IHC staining of human renal cell carcinoma using 12615-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NIFK Polyclonal antibody (12615-1-AP)

    IF Staining of MCF-7 using 12615-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 12615-1-AP (NIFK antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using NIFK Polyclonal antibody (12615-1-AP)

    FC experiment of MCF-7 using 12615-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug NIFK Polyclonal antibody (12615-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12615-1-AP targets NIFK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIFK fusion protein Ag3284
Full Name MKI67 (FHA domain) interacting nucleolar phosphoprotein
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC024238
Gene Symbol NIFK
Gene ID (NCBI) 84365
RRIDAB_2142384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NIFK antibody 12615-1-APDownload protocol
IHC protocol for NIFK antibody 12615-1-APDownload protocol
IF protocol for NIFK antibody 12615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article