Anticorps Monoclonal anti-Alpha Tubulin

Alpha Tubulin Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, rat, souris et plus (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

835

CloneNo.

1E4C11

Avis (9)

N° de cat : 66031-1-Ig

Synonymes

alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, tubulin, Tubulin alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 66031-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66031-1-Ig

  IP Result of anti-alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human tonsillitis using 66031-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66031-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66031-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66031-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsillitis using 66031-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66031-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66031-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green).

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  IF Staining of MCF-7 using 66031-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green).

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  IF Staining of rat brain using 66031-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66031-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red).

• 

  IF Staining of HepG2 using 66031-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

• 

  FC experiment of HeLa using 66031-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 754 publications below
• IHC: See 1 publications below
• IF: See 63 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

66031-1-Ig cible Alpha Tubulin dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poisson-zèbre, poulet, singe, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: TUBA1B
• Identification du gène (NCBI): 10376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination¹, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).

Publications

Species	Application	Title
	WB	Nature; Structural basis for FGF hormone signalling; Authors - Lingfeng Chen
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human,monkey	WB,IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Signal Transduct Target Ther; FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth; Authors - Wenxia Wei
human	WB	Nat Microbiol; PMID: 31649358; Authors - Tingting Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chiara (Verified Customer) (10-02-2023)

This antibody worked quite well in our immunofluorescence experiments

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human primary skin fibroblasts

Alpha Tubulin Antibody Immunofluorescence validation (1:1000 dilution) in human primary skin fibroblasts (Cat no:66031-1-Ig)

FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:2000

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Bone Marrow MSCs

FH

Amy (Verified Customer) (08-10-2023)

Clean strong bands for western blot on HEK293T lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:50,000
• Cell Tissue Type: HEK293T

FH

Kalin (Verified Customer) (03-12-2022)

I think this antibody works great for western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Sea urchin lysate

FH

Silvia (Verified Customer) (02-18-2022)

This antibody worked quite well in our western blotting and it comes at a great price.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

FH

Azita (Verified Customer) (06-02-2021)

Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Alpha Tubulin Monoclonal Antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mice spinal cord tissues

FH

Scooby (Verified Customer) (10-05-2020)

happy

• Applications: Immunofluorescence
• Cell Tissue Type: n/a

FH

Lauren (Verified Customer) (10-05-2020)

thanks

• Applications: Western Blot

FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HeLa cells

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