Anticorps Monoclonal anti-Phospho-TDP43 (Ser409/410)

Phospho-TDP43 (Ser409/410) Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

1A2C1

Avis (1)

N° de cat : 66318-1-Ig

Synonymes

ALS10, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43

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Galerie de données de validation

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  WB analysis of HeLa using 66318-1-Ig

  Untreated and EA treated HeLa cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) Antibody) at dilution of 1:850 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66318-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66318-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410)antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66318-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66318-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à l'AE, cellules C6, cellules HEK-293, cellules Neuro-2a, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

66318-1-Ig cible Phospho-TDP43 (Ser409/410) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 45-50 kDa, 35 kDa
• Numéro d’acquisition GenBank: NM_007375
• Symbole du gène: TARDBP
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.

Publications

Species	Application	Title
human	IF	Mol Cell; Stress Induces Dynamic, Cytotoxicity-Antagonizing TDP-43 Nuclear Bodies via Paraspeckle LncRNA NEAT1-Mediated Liquid-Liquid Phase Separation.; Authors - Chen Wang
human	IHC	Acta Neuropathol Commun; Ex vivo MRI atlas of the human medial temporal lobe: characterizing neurodegeneration due to tau pathology.; Authors - Sadhana Ravikumar
		PLoS Genet; Progressive ataxia of Charolais cattle highlights a role of KIF1C in sustainable myelination.; Authors - Amandine Duchesne
human	IHC	Eur J Neurol; SOD1 p.D12Y variant is associated with amyotrophic lateral sclerosis/distal myopathy spectrum.; Authors - G Tasca
human	IHC	Neuroimage Clin; Ex vivo MRI and histopathology detect novel iron-rich cortical inflammation in frontotemporal lobar degeneration with tau versus TDP-43 pathology.; Authors - M Dylan Tisdall
human	WB,IF	Mol Cell Biol; The N-termini of TAR DNA-binding protein-43 (TDP43) C-terminal fragments influence degradation, aggregation propensity and morphology.; Authors - Yasar Arfat T Kasu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alejandro (Verified Customer) (04-08-2020)

Good product

• Applications: Immunohistochemistry

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