Anticorps Polyclonal de lapin anti-YME1L1

• Phare
• Validé par KD/KO

YME1L1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

71

Avis (1)

N° de cat : 11510-1-AP

Synonymes

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

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Galerie de données de validation

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  WB analysis using 11510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11510-1-AP

  WB result of YME1L1 antibody (11510-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells.

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  IP experiment of HeLa using 11510-1-AP

  IP result of anti-YME1L1 (IP:11510-1-AP, 4ug; Detection:11510-1-AP 1:500) with HeLa cells lysate 1280 ug.

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  IF Staining of HepG2 using 11510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YME1L1 antibody (11510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 61 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11510-1-AP cible YME1L1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YME1L1 Protéine recombinante Ag2069
• Nom complet: YME1-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 716 aa, 80 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC023507
• Symbole du gène: YME1L1
• Identification du gène (NCBI): 10730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Publications

Species	Application	Title
mouse	WB	Cell Metab; C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.; Authors - Tao Wang ; KD Validated
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	IF	Mol Cell; Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress.; Authors - Raul Jobava

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (03-08-2022)

incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

YME1L1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11510-1-AP)

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