Presenilin-1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

Presenilin-1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 16163-1-AP

Synonyms

Presenilin-1-Specific, PSEN1, PSNL1, PS1, PS-1, AD3, EC:3.4.23.-, Presenilin-1, Presenilin-1 CTF subunit, Presenilin-1 CTF12, Presenilin-1 NTF subunit

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Validation Data Gallery

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  WB analysis using 16163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16163-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16163-1-AP

  IP result of anti-Presenilin-1-Specific (IP:16163-1-AP, 4ug; Detection:16163-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 16163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COS-7 cells, human kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human prostate cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 1 publications below

Product Information

16163-1-AP targets Presenilin-1-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: presenilin 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 21 kDa, 53 kDa
• GenBank Accession Number: NM_000021
• Gene Symbol: PSEN1
• Gene ID (NCBI): 5663
• RRID: AB_2237805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human,mouse	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
human	WB	Cell Commun Signal; Presenilin1 exerts antiproliferative effects by repressing the Wnt/β-catenin pathway in glioblastoma.; Authors - Wei Yang ; KD Validated
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

Presenilin-1-Specific Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:16163-1-AP)