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Presenilin-1-Specific Polyclonal antibody
Presenilin-1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey and More (1)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16163-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COS-7 cells, human kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 17 publications below |
IHC | See 1 publications below |
Product Information
16163-1-AP targets Presenilin-1-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | presenilin 1 |
Calculated Molecular Weight | 53 kDa |
Observed Molecular Weight | 21 kDa, 53 kDa |
GenBank Accession Number | NM_000021 |
Gene Symbol | PSEN1 |
Gene ID (NCBI) | 5663 |
RRID | AB_2237805 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Presenilin-1-Specific antibody 16163-1-AP | Download protocol |
IHC protocol for Presenilin-1-Specific antibody 16163-1-AP | Download protocol |
IP protocol for Presenilin-1-Specific antibody 16163-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression | ||
Brain MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation. | ||
Cell Rep CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease. | ||
J Neuroinflammation Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice. | ||
Cell Commun Signal Presenilin1 exerts antiproliferative effects by repressing the Wnt/β-catenin pathway in glioblastoma.
| ||
Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xiaoyu (Verified Customer) (02-07-2024) | Good signal
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