Anticorps Polyclonal de lapin anti-TRAPPC9, NIBP

• Phare
• Validé par KD/KO

TRAPPC9, NIBP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 16014-1-AP

Synonymes

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRS120, Tularik gene 1 protein

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Galerie de données de validation

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  WB analysis of mouse brain using 16014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16014-1-AP

  IP result of anti-TRAPPC9, NIBP (IP:16014-1-AP, 4ug; Detection:16014-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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  IHC staining of human kidney using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 16014-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRAPPC9, NIBP antibody (16014-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16014-1-AP cible TRAPPC9, NIBP dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAPPC9, NIBP Protéine recombinante Ag8791
• Nom complet: trafficking protein particle complex 9
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 128-140 kDa
• Numéro d’acquisition GenBank: BC006206
• Symbole du gène: NIBP/Trappc9
• Identification du gène (NCBI): 83696
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAPPC9, also named KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cell differentiation and play a role in vesicular transport from the endoplasmic reticulum to the Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. Western blotting indicates the molecular weight of TRAPPC9 is 130-140 kDa, and 250 kDa may be detected with extensive posttranslational modification of TRAPPC9.

Publications

Species	Application	Title
human	IF	EMBO J; The TRAPP complex mediates secretion arrest induced by stress granule assembly.; Authors - Francesca Zappa
human	IF	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
mouse,zebrafish	WB,IF	Int J Biol Sci; Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice; Authors - Min Hu
mouse	WB	EMBO Rep; TRAPPC1 is essential for the maintenance and differentiation of common myeloid progenitors in mice; Authors - Yanan Xu
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca ; KD Validated
human	WB,IHC	Tumour Biol; NIK- and IKKβ-binding protein promotes colon cancer metastasis by activating the classical NF-κB pathway and MMPs.; Authors - Mengbin Qin

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