TRAPPC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAPPC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15555-1-AP

Synonyms

CDABP0066, BET3 homolog, BET3

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Validation Data Gallery

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  WB analysis of HEK-293 using 15555-1-AP

  WB result of TRAPPC3 antibody (15555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC3 transfected HEK-293 cells.

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  WB analysis of mouse liver using 15555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 15555-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15555-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15555-1-AP

  IP result of anti-TRAPPC3 (IP:15555-1-AP, 3ug; Detection:15555-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human placenta using 15555-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15555-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15555-1-AP

  Immunofluorescent analysis of Hela cells, using TRAPPC3 antibody 15555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, PC-3 cells, HEK-293 cells, mouse small intestine tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

15555-1-AP targets TRAPPC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPPC3 fusion protein Ag7924
• Full Name: trafficking protein particle complex 3
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC007662
• Gene Symbol: TRAPPC3
• Gene ID (NCBI): 27095
• RRID: AB_2208142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC3 (trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines. (15728249, 21273506, 23394947)

Publications

Species	Application	Title
human	WB	Cell; A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.; Authors - Bassik Michael C MC
human	WB	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
mouse	WB	Int J Biol Sci; Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice; Authors - Min Hu
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca
human	WB	Signal Transduct Target Ther; RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy.; Authors - Yalan Lu
human	WB	Metabolites; Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response; Authors - Yun Wu