TRAPPC2 Polyclonal antibody

TRAPPC2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12484-1-AP

Synonyms

TRAPPC2B, TRAPPC2.19, Trafficking protein particle complex subunit 2B, SEDLP1, MIP-2A

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Validation Data Gallery

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  WB analysis of human brain using 12484-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12484-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12484-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12484-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12484-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12484-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 12484-1-AP

  IP result of anti-TRAPPC2 (IP:12484-1-AP, 3ug; Detection:12484-1-AP 1:500) with PC-3 cells lysate 2800ug.

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  IHC staining of human stomach using 12484-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12484-1-AP (TRAPPC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12484-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12484-1-AP (TRAPPC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, human liver tissue, human lung tissue, mouse brain tissue, mouse thymus tissue, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12484-1-AP targets TRAPPC2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPPC2 fusion protein Ag3201
• Full Name: trafficking protein particle complex 2
• Calculated Molecular Weight: 140 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC016915
• Gene Symbol: TRAPPC2
• Gene ID (NCBI): 6399
• RRID: AB_2208136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca
rat	WB	iScience; Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and white matter development; Authors - Tiantian Ma