Anticorps Polyclonal de lapin anti-TH

TH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

104

Avis (4)

N° de cat : 25859-1-AP

Synonymes

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

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Galerie de données de validation

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  WB analysis using 25859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25859-1-AP

  IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:1000) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 25859-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 25859-1-AP (TH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 63 publications below
• IHC: See 33 publications below
• IF: See 39 publications below
• ELISA: See 1 publications below

Informations sur le produit

25859-1-AP cible TH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, souris, Gerbille
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TH Protéine recombinante Ag23075
• Nom complet: tyrosine hydroxylase
• Masse moléculaire calculée: 528 aa, 59 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC104967
• Symbole du gène: TH
• Identification du gène (NCBI): 7054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.; Authors - Yan-Ting Chen
mouse	IF	Nat Commun; NAc-VTA circuit underlies emotional stress-induced anxiety-like behavior in the three-chamber vicarious social defeat stress mouse model.; Authors - Guangjian Qi
mouse	IF	Nat Commun; Locus coeruleus-CA1 projections are involved in chronic depressive stress-induced hippocampal vulnerability to transient global ischaemia.; Authors - Qian Zhang
mouse	WB	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
mouse	IHC	Acta Biomater; Biomaterial based strategies to reconstruct the nigrostriatal pathway in organotypic slice co-cultures.; Authors - Buket Ucar
mouse	IHC	Redox Biol; Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.; Authors - Zhuqing Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

This antibody worked well for immunolabeling on mouse brain tissue sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse brain

FH

Raphael (Verified Customer) (08-02-2021)

This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Rat brain

TH Antibody Western Blot validation (1/2000 dilution) in Rat brain (Cat no:25859-1-AP)

FH

Teresa (Verified Customer) (11-03-2017)

Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: wildtype mouse brain

TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)

FH

Teresa (Verified Customer) (10-16-2017)

Signal was strong and specific. Could probably dilute further.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse brain

TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)

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