Anticorps Polyclonal de lapin anti-TFF2

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (1)

N° de cat : 13681-1-AP

Synonymes

SML1, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin, SP

---

Galerie de données de validation

• 

  WB analysis of human stomach using 13681-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13681-1-AP

  human stomach sample were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13681-1-AP

  Human urine tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse stomach using 13681-1-AP

  IP result of anti-TFF2 (IP:13681-1-AP, 4ug; Detection:13681-1-AP 1:600) with mouse stomach tissue lysate 4000ug.

• 

  IHC staining of human stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse stomach using 13681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse stomach using 13681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse stomach using 13681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu d'estomac humain, échantillon d'urine humaine
• Résultats positifs en IP: tissu d'estomac de souris
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de l'estomac humain, tissu d'estomac de souris, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'estomac de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 25 publications below
• IF: See 19 publications below

Informations sur le produit

13681-1-AP cible TFF2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris, Gerbille
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFF2 Protéine recombinante Ag4537
• Nom complet: trefoil factor 2
• Masse moléculaire calculée: 129 aa, 14 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC032820
• Symbole du gène: TFF2
• Identification du gène (NCBI): 7032
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

Publications

Species	Application	Title
mouse	IF	Nature; A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.; Authors - Daniel T Montoro
mouse	IF	Nat Genet; Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development.; Authors - Lijiang Fei
mouse	IF,IHC	Nat Cell Biol; Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach.; Authors - Marc Leushacke
human,mouse	IHC,IF	Nat Cell Biol; A tumour-resident Lgr5+ stem-cell-like pool drives the establishment and progression of advanced gastric cancers.; Authors - A Fatehullah
human	IF	Gut; Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing.; Authors - Min Zhang
mouse	IF	Gastroenterology; Induction of gastric cancer by successive oncogenic activation in the corpus.; Authors - Daisuke Douchi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hongxue (Verified Customer) (05-13-2022)

WB works.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

Produits associés

Trefoil factor 1 Polyclonal antibody

13734-1-AP

TAC1 Polyclonal antibody

13839-1-AP

PPAR Gamma Polyclonal antibody

16643-1-AP

P53 Monoclonal antibody

60283-1-Ig

CXCR4 Polyclonal antibody

11073-2-AP

Gastrin Polyclonal antibody

18143-1-AP