Anticorps Polyclonal de lapin anti-Syntaxin 11

• Phare
• Validé par KD/KO

Syntaxin 11 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 13301-1-AP

Synonymes

Syntaxin-11, STX11

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Galerie de données de validation

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  WB analysis using 13301-1-AP

  Jurkat cells lysates were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13301-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 13301-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 13301-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13301-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13301-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13301-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 13301-1-AP

  IP result of anti-Syntaxin 11 (IP:13301-1-AP, 4ug; Detection:13301-1-AP 1:1000) with DU 145 cells lysate 1280 ug.

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  IHC staining of human lymphoma using 13301-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13301-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules DU 145, cellules L02, tissu de muscle squelettique humain, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IP: cellules DU 145,
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

13301-1-AP cible Syntaxin 11 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntaxin 11 Protéine recombinante Ag4097
• Nom complet: syntaxin 11
• Masse moléculaire calculée: 287 aa, 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC033519
• Symbole du gène: Syntaxin 11
• Identification du gène (NCBI): 8676
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286).

Publications

Species	Application	Title
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
rat	WB,IF	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
human	WB,IF, IP	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB, IHC	J Cell Sci; Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis.; Authors - Na-Ryum Bin
human	WB	Front Immunol; Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.; Authors - Jamie A Lopez

Produits associés

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15312-1-AP

SNAP23 Polyclonal antibody

10825-1-AP

Perforin Polyclonal antibody

14580-1-AP

Syntaxin 12 Polyclonal antibody

14259-1-AP