STXBP2 Polyclonal antibody

STXBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15312-1-AP

Synonyms

Hunc18b, MUNC18 2, pp10122, STXBP2, STXBP2 protein, syntaxin binding protein 2, UNC18 2, UNC18B

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Validation Data Gallery

• 

  WB analysis of K-562 using 15312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15312-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15312-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human lung cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

15312-1-AP targets STXBP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STXBP2 fusion protein Ag7537
• Full Name: syntaxin binding protein 2
• Calculated Molecular Weight: 593 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC002869
• Gene Symbol: STXBP2
• Gene ID (NCBI): 6813
• RRID: AB_2196821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.

Publications

Species	Application	Title
	IF	Nanomicro Lett; Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling; Authors - Junji Ren
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
human	WB	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB	Front Immunol; An N-Terminal Missense Mutation in STX11 Causative of FHL4 Abrogates Syntaxin-11 Binding to Munc18-2.; Authors - Martha-Lena Müller
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
	WB	Int J Hematol; Molecular analysis of the novel L243R mutation in STXBP2 reveals impairment of degranulation activity.; Authors - Laura Viñas-Giménez

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