- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-SOCS3
SOCS3 Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (4)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 14025-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HeLa, |
| Résultats positifs en IP | cellules K-562 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 4 publications below |
| WB | See 56 publications below |
| IHC | See 11 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
14025-1-AP cible SOCS3 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | rat, Humain, porc, poulet, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | SOCS3 Protéine recombinante Ag5170 |
| Nom complet | suppressor of cytokine signaling 3 |
| Masse moléculaire calculée | 25 kDa |
| Poids moléculaire observé | 25-30 kDa |
| Numéro d’acquisition GenBank | BC060858 |
| Symbole du gène | SOCS3 |
| Identification du gène (NCBI) | 9021 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for SOCS3 antibody 14025-1-AP | Download protocol |
| IP protocol for SOCS3 antibody 14025-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes | ||
Cell Death Differ TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation. | ||
Kidney Int Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury. | ||
Oncoimmunology Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment. | ||
PLoS Pathog Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication | ||
