Anticorps Polyclonal de lapin anti-SMYD3

• Phare
• Validé par KD/KO

SMYD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 12011-1-AP

Synonymes

ZMYND1, Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, Histone-lysine N-methyltransferase SMYD3, EC:2.1.1.354

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Galerie de données de validation

• 

  WB analysis of mouse brain using 12011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12011-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12011-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12011-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12011-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12011-1-AP

  IP result of anti-SMYD3 (IP:12011-1-AP, 4ug; Detection:12011-1-AP 1:500) with COLO 320 cells lysate 1280ug.

• 

  IHC staining of human breast cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 12011-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SMYD3 antibody (12011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

12011-1-AP cible SMYD3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMYD3 Protéine recombinante Ag2624
• Nom complet: SET and MYND domain containing 3
• Masse moléculaire calculée: 428aa,49 kDa; 369aa,42 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC031010
• Symbole du gène: SMYD3
• Identification du gène (NCBI): 64754
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) . SMYD3 exists some isoforms with MV 49, 43 and 30 kDa.

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.; Authors - Fen Long ; KD Validated
human	WB	Carcinogenesis; miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.; Authors - Furuta Mayuko M
rat	WB,IHC	CNS Neurosci Ther; Integration of Methylation and Gene Expression Deciphered Candidate Biomarkers DAB2IP and SMYD3 in Delayed Encephalopathy After Carbon Monoxide Poisoning; Authors - Hongyi Yan
human	WB	Int J Androl; Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.; Authors - Lambrot R R
human	WB	Med Oncol; PMID: 20957523; Authors - Ren Tian-nian TN ; KD Validated
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in the HEK293T cells with human SMYD3 overexpression.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: HEK293T cells

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