Anticorps Monoclonal anti-SMN-Exon7

SMN-Exon7 Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3A8G11

N° de cat : 60255-1-Ig

Synonymes

Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMN-Exon7, SMNT, Survival motor neuron protein, T BCD541

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Galerie de données de validation

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  WB analysis of HEK-293 using 60255-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60255-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60255-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 60255-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60255-1-Ig cible SMN-Exon7 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMN-Exon7 Protéine recombinante Ag16615
• Nom complet: survival of motor neuron 1, telomeric
• Masse moléculaire calculée: 294 aa, 32 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC062723
• Symbole du gène: SMN1
• Identification du gène (NCBI): 6606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.

Publications

Species	Application	Title
	WB	Science; Base editing rescue of spinal muscular atrophy in cells and in mice; Authors - Mandana Arbab

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