Anticorps Polyclonal de lapin anti-SLC7A5

• Phare
• Validé par KD/KO

SLC7A5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

16

N° de cat : 13752-1-AP

Synonymes

D16S469E, CD98LC, CD98 light chain, 4F2LC, 4F2 light chain

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Galerie de données de validation

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  WB analysis of mouse lung using 13752-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13752-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13752-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer de l'estomac humain, tissu hépatique de souris, tissu pulmonaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

13752-1-AP cible SLC7A5 dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris, Vache
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC7A5 Protéine recombinante Ag4694
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC039692
• Symbole du gène: SLC7A5
• Identification du gène (NCBI): 8140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang
human	IHC	J Nucl Med; PMID: 29700126; Authors - Deep Hathi
mouse	WB	Mol Metab; Resistance exercise enhances long-term mTORC1 sensitivity to leucine; Authors - Gommaar D'Hulst
human	WB	ACS Chem Neurosci; Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism.; Authors - Li Gao
human	WB,IHC	J Biol Chem; GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.; Authors - Keshuo Ding
human	IHC	Br J Cancer; ASCT2 (SLC1A5)-dependent glutamine uptake is involved in the progression of head and neck squamous cell carcinoma.; Authors - Ze Zhang

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