CD98/SLC3A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD98/SLC3A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

45

Cat no : 15193-1-AP

Synonyms

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

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Validation Data Gallery

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  WB analysis of HeLa using 15193-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15193-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells
• Positive IHC detected in: rat testis tissue, mouse testis tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 37 publications below
• IHC: See 7 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Product Information

15193-1-AP targets CD98/SLC3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD98/SLC3A2 fusion protein Ag7280
• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 85-94 kDa, 120-130 kDa
• GenBank Accession Number: BC001061
• Gene Symbol: CD98
• Gene ID (NCBI): 6520
• RRID: AB_2254909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB,IHC	Sci Transl Med; Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.; Authors - Kana Hasegawa
mouse	IF	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	IF	J Allergy Clin Immunol; Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function.; Authors - Siddhartha S Saha
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai
mouse	WB	Cell Rep; Brown adipocyte ATF4 activation improves thermoregulation and systemic metabolism; Authors - Esther Paulo