CD147 Polyclonal antibody

CD147 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11989-1-AP

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF



Tested Applications

Positive WB detected inhuman liver tissue, Jurkat cells, mouse liver tissue, mouse heart tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11989-1-AP targets CD147 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD147 fusion protein Ag2619
Full Name basigin (Ok blood group)
Calculated Molecular Weight 385 aa, 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC009040
Gene Symbol CD147
Gene ID (NCBI) 682
RRIDAB_2290597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).

Protocols

Product Specific Protocols
WB protocol for CD147 antibody 11989-1-APDownload protocol
IHC protocol for CD147 antibody 11989-1-APDownload protocol
IP protocol for CD147 antibody 11989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover

Authors - Michael J Podolsky
humanWB

J Clin Invest

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

Authors - Huilun Helen Wang
mouseCoIP

Cell Mol Immunol

CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.

Authors - Ruo Chen
mouseIHC

Acta Pharmacol Sin

124I-labeled anti-CD147 antibody for noninvasive detection of CD147-positive pan-cancers: construction and preclinical studies

Authors - Xiao-Kun Ma
humanWB

Elife

Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes

Authors - Abhishek Jamwal
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mohammed (Verified Customer) (08-19-2020)

Stains the sperm tail very nicely.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: (1:200)
  • Cell Tissue Type: Testis