CD147 Polyclonal antibody

CD147 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

35

Reviews (1)

Cat no : 11989-1-AP

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Validation Data Gallery

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  WB analysis of human liver using 11989-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11989-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11989-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11989-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11989-1-AP

  IP result of anti-CD147 (IP:11989-1-AP, 4ug; Detection:11989-1-AP 1:10000) with HEK-293 cells lysate 1200 ug.

• 

  IHC staining of human lung cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11989-1-AP (CD147 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human liver tissue, Jurkat cells, mouse liver tissue, mouse heart tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

11989-1-AP targets CD147 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD147 fusion protein Ag2619
• Full Name: basigin (Ok blood group)
• Calculated Molecular Weight: 385 aa, 42 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC009040
• Gene Symbol: CD147
• Gene ID (NCBI): 682
• RRID: AB_2290597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).

Publications

Species	Application	Title
human	WB	Nat Commun; Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover; Authors - Michael J Podolsky
human	WB	J Extracell Vesicles; Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles; Authors - Liqiao Hu
human	WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
mouse	CoIP	Cell Mol Immunol; CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.; Authors - Ruo Chen
mouse	IHC	Acta Pharmacol Sin; 124I-labeled anti-CD147 antibody for noninvasive detection of CD147-positive pan-cancers: construction and preclinical studies; Authors - Xiao-Kun Ma
human	WB	Elife; Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes; Authors - Abhishek Jamwal

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Stains the sperm tail very nicely.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis