Anticorps Polyclonal de lapin anti-SECISBP2

• Phare
• Validé par KD/KO

SECISBP2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12798-1-AP

Synonymes

SBP2, SECIS binding protein 2, SECISBP2

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Galerie de données de validation

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  WB analysis of HeLa using 12798-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12798-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12798-1-AP

  IP result of anti-SECISBP2 (IP:12798-1-AP, 3ug; Detection:12798-1-AP 1:600) with mouse testis tissue lysate 8000ug.

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  IF Staining of HeLa using 12798-1-AP

  Immunofluorescent analysis of HeLa cells using 12798-1-AP (SECISBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu rénal humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

12798-1-AP cible SECISBP2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SECISBP2 Protéine recombinante Ag3541
• Nom complet: SECIS binding protein 2
• Masse moléculaire calculée: 854 aa, 95 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC036109
• Symbole du gène: SECISBP2
• Identification du gène (NCBI): 79048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Selenium (Se) is an essential trace element required for the biosynthesis of selenoproteins, and selenocysteine insertion sequence (SECIS) binding protein 2 (SECISBP2, or SBP2) represents a key trans-acting factor for the cotranslational insertion of selenocysteine into selenoproteins. Defects in SBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) associated with a reduction in type II iodothyronine deiodinase activity. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Cells depleted of SBP2 have increased levels of ROS, which lead to cellular oxidative stress manifested as DNA lesions, stress granules, and lipid peroxidation, induction of caspase- and cytochrome c-dependent apoptosis, indicating that SBP2 is required for protection against ROS-induced cellular damage and cell survival.

Publications

Species	Application	Title
human	WB	Science; SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding.; Authors - Hsiu-Chuan Lin
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
human	WB	Nucleic Acids Res; Identification of a novel endogenous long non-coding RNA that inhibits selenoprotein P translation.; Authors - Yuichiro Mita
human	WB,IF	Free Radic Biol Med; Selective up-regulation of human selenoproteins in response to oxidative stress.; Authors - Touat-Hamici Zahia
mouse	WB	Endocrinology; Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.; Authors - Jiao Fu ; KO Validated
human	WB,IF	J Biol Chem; Interplay between Selenium Levels, Selenoprotein Expression and Replicative Senescence in WI-38 Human Fibroblasts.; Authors - Yona Legrain

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