SEPN1 Polyclonal antibody

SEPN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 55333-1-AP

Synonyms

MDRS1, RSMD1, RSS, SELENON, Selenoprotein N, selenoprotein N, 1, SELN, SEPN1

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Validation Data Gallery

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  WB analysis of A549 using 55333-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse uterus using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse uterus tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below

Product Information

55333-1-AP targets SEPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: selenoprotein N, 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_020451
• Gene Symbol: SEPN1
• Gene ID (NCBI): 57190
• RRID: AB_2881308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Changes and related factors of blood CCN1 levels in diabetic patients; Authors - Zhao-Yu Xiang
human	WB	Antioxidants (Basel); Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.; Authors - Dian-Guang Zhang
mouse	WB	Sci Rep; Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins.; Authors - Jiayong Tang
mouse	WB	Mar Drugs; Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.; Authors - Chih-Hung Guo
mouse	WB	Front Nutr; Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation.; Authors - Arlene C P Kiyohara
	WB	Br J Nutr; Dietary selenium (Se) sources differentially regulate Se concentration, mRNA and protein expression of representative selenoproteins in various tissues of yellow catfish Pelteobagrus fulvidraco.; Authors - Xiao-Jian Xu