SOD2 Polyclonal antibody

SOD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

321

Reviews (1)

Cat no : 24127-1-AP

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

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Validation Data Gallery

• 

  WB analysis using 24127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24127-1-AP (SOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 24127-1-AP

  IP result of anti-SOD2 (IP:24127-1-AP, 4ug; Detection:24127-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24127-1-AP (SOD2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24127-1-AP (SOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24127-1-AP (SOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of HUVEC cells using 24127-1-AP (SOD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (24127-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 24127-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (24127-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (24127-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (24127-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 292 publications below
• IHC: See 28 publications below
• IF: See 28 publications below
• IP: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 3 publications below

Product Information

24127-1-AP targets SOD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOD2 fusion protein Ag21388
• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• RRID: AB_2879437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a 

mitochondrial enzyme that dismutates superoxide ions.

What is the molecular weight of SOD2? Is SOD2 post-translationally modified?

The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be 

post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2 

increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068), 

while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).

What is the subcellular localization of superoxide dismutases?

SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is 

predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and 

nucleus (PMID: 11507097), while SOD3 localizes extracellularly.

What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?

Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial 

matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals 

to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses 

they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases 

and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from 

oxidative damage (PMID: 19228881).

What is the tissue expression pattern of SOD2?

SOD2 is ubiquitously expressed.

Can SOD2 be used as a marker of oxidative stress?

SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2 

activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress 

because its expression is not always altered by oxygen stress. 

Publications

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB	Adv Sci (Weinh); IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor Metastasis; Authors - Guoqing Cai
human	WB	Nat Commun; Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.; Authors - Haohao Huang
mouse	WB	Redox Biol; miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation; Authors - Xiaofei Wang
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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