Anticorps Polyclonal de lapin anti-RSPO3

• Phare
• Validé par KD/KO

RSPO3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 17193-1-AP

Synonymes

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

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Galerie de données de validation

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  WB analysis using 17193-1-AP

  mouse small intestine were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 17193-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 17193-1-AP

  IP result of anti-RSPO3 (IP:17193-1-AP, 4ug; Detection:17193-1-AP 1:300) with human placenta tissue lysate 2800ug.

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  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris, cellules HEK-293 transfectées
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17193-1-AP cible RSPO3 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSPO3 Protéine recombinante Ag9899
• Nom complet: R-spondin 3 homolog (Xenopus laevis)
• Masse moléculaire calculée: 272 aa, 31 kDa
• Poids moléculaire observé: 30-40 kDa
• Numéro d’acquisition GenBank: BC022367
• Symbole du gène: RSPO3
• Identification du gène (NCBI): 84870
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Publications

Species	Application	Title
mouse	WB	Nat Commun; R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.; Authors - Teng Han
human	WB	Proc Natl Acad Sci U S A; RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.; Authors - Kendra S Carmon
mouse	WB,IF	Cell Death Dis; Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury; Authors - Ting-Tao Liu ; KD Validated
human	WB	Cardiovasc Res; Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis.; Authors - Bhupesh Singla
rat	WB	Heliyon; Astragalus polysaccharide improves diabetic ulcers by promoting M2-polarization of macrophages to reduce excessive inflammation via the β-catenin/ NF-κB axis at the late phase of wound-healing; Authors - Zhang Zhen
human	IHC	Am J Physiol Cell Physiol; R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.; Authors - Zhilong Chen

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