Anticorps Polyclonal de lapin anti-CD45

CD45 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

91

Avis (3)

N° de cat : 20103-1-AP

Synonymes

LCA, L CA, GP180, EC:3.1.3.48, CD45R

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Galerie de données de validation

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  WB analysis using 20103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20103-1-AP (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 20103-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20103-1-AP (CD45 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 20103-1-AP

  IP result of anti-CD45 (IP:20103-1-AP, 4ug; Detection:20103-1-AP 1:300) with mouse spleen tissue lysate 4800ug.

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  IHC staining of human colon using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20103-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 20103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD45 antibody (20103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 20103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD45 antibody (20103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu de thymus de rat, tissu de thymus de souris, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu d'amygdalite humain, tissu d'appendicite humain, tissu de cancer du foie humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 42 publications below
• IF: See 34 publications below
• FC: See 6 publications below

Informations sur le produit

20103-1-AP cible CD45 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD45 Protéine recombinante Ag13712
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 1304 aa, 147 kDa
• Poids moléculaire observé: 180-220 kDa
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596).  

What is the molecular weight of CD45?

The molecular weight of CD45 is a 180-240 kDa. 

What are the isoforms of CD45?

Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). 

What is the tissue specificity of CD45?

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). 

What are the post-translational modifications of CD45?

CD45 can be heavily N- and O-glycosylated (PMID: 1702721). 

What is CD45's involvement in disease?

Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).

Publications

Species	Application	Title
mouse	IHC	Cell Res; SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses; Authors - Shufen Li
mouse	IF	Hepatology; Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.; Authors - Wu Yang
	IF	Nat Commun; Brain-to-cervical lymph node signaling after stroke.; Authors - Elga Esposito
human	IF	Cell Death Differ; SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination.; Authors - Zhengrong Zhang
human		Theranostics; COL4A2 in the tissue-specific extracellular matrix plays important role on osteogenic differentiation of periodontal ligament stem cells.; Authors - Yi Wen
human	IF	Int J Nanomedicine; A TSHR-Targeting Aptamer in Monocytes Correlating with Clinical Activity in TAO; Authors - Jiamin Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandru (Verified Customer) (11-13-2023)

Works great on paraffin embedded tissue section (after antigen retrival!)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse ventricle

FH

Kenzo (Verified Customer) (01-09-2023)

This antibody worked well as a hematopoietic cell marker for IF on tissue sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse tonsil

FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: Prostate

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