ZAP70 Polyclonal antibody

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  WB analysis of Jurkat using 22207-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 22207-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 22207-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22207-1-AP

  IP result of anti-ZAP70 (IP:22207-1-AP, 4ug; Detection:22207-1-AP 1:8000) with Jurkat cells lysate 1320 ug.

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  IHC staining of human tonsillitis using 22207-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22207-1-AP (ZAP70 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22207-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22207-1-AP (ZAP70 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 22207-1-AP

  Immunofluorescent analysis of RAW 264.7 cells using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ZAP70 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK, STD

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Tested Applications

• Positive WB detected in: Jurkat cells, RAW 264.7 cells, RAW264.7
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22207-1-AP targets ZAP70 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17527

  Product name: Recombinant human ZAP70 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 109-267 aa of BC039039

  Sequence: LVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQL

• Full Name: zeta-chain (TCR) associated protein kinase 70kDa
• Calculated Molecular Weight: 619 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039039
• Gene Symbol: ZAP70
• Gene ID (NCBI): 7535
• ENSEMBL Gene ID: ENSG00000115085
• RRID: AB_2879029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43403
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.