ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 22207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, RAW264.7
Positive IP detected inJurkat cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22207-1-AP targets ZAP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag17527 Predict reactive species
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated Molecular Weight 619 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_2879029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43403
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZAP70 antibody 22207-1-APDownload protocol
IF protocol for ZAP70 antibody 22207-1-APDownload protocol
IP protocol for ZAP70 antibody 22207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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