Anticorps Polyclonal de lapin anti-RIPK1-Specific

• Phare
• Validé par KD/KO

RIPK1-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (3)

Applications

WB, IHC, IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

111

Avis (1)

N° de cat : 17519-1-AP

Synonymes

RIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1

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Galerie de données de validation

• 

  WB analysis using 17519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17519-1-AP (RIPK1-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 17519-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17519-1-AP (RIPK1-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse small intestine using 17519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17519-1-AP (RIPK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu d'intestin grêle de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 99 publications below
• IHC: See 13 publications below
• IF: See 14 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

17519-1-AP cible RIPK1-Specific dans les applications de WB, IHC, IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, levure, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_003804
• Symbole du gène: RIPK1
• Identification du gène (NCBI): 8737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397).

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.; Authors - Ziqiao Yuan
mouse	WB	Circ Res; AirnRegulates Igf2bp2 Translation in Cardiomyocytes.; Authors - Mohammed R Hosen
mouse	WB,IP	Nat Commun; Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis.; Authors - Jiefei Geng
mouse	IHC	Sci Total Environ; Evaluation of 6-PPD quinone toxicity on lung of male BALB/c mice by quantitative proteomics; Authors - Wenmiao He
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
mouse	WB	Environ Pollut; Polystyrene nanoplastics and cadmium co-exposure aggravated cardiomyocyte damage in mice by regulating PANoptosis pathway; Authors - Jiali Ye

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yuan (Verified Customer) (01-07-2020)

This RIPK-1 antibody was tried on the MCF7 tamoxifen resistant (5uM) cell line, the 74 kDa band was very faint, but the 45 kDa band is clear. In addition, some unspecific bands near 45 kDa showed up too.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7-TR5

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