Anticorps Polyclonal de lapin anti-RAB7A

• Phare
• Validé par KD/KO

RAB7A Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

44

Avis (6)

N° de cat : 55469-1-AP

Synonymes

PRO2706, RAB7, RAB7A, Ras related protein Rab 7a

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Galerie de données de validation

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  WB analysis using 55469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55469-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 55469-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55469-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 55469-1-AP

  IP result of anti-RAB7A (IP:55469-1-AP, 4ug; Detection:55469-1-AP 1:1000) with SH-SY5Y cells lysate 3360 ug.

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  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of A431 using 55469-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB7A (55469-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules L-929, cellules A431, cellules C6, cellules NIH/3T3, cellules RAW 264.7, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de muscle squelettique humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

55469-1-AP cible RAB7A dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB7A, member RAS oncogene family
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: NM_004637
• Symbole du gène: RAB7A
• Identification du gène (NCBI): 7879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
Zebrafish,monkey		Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
		Nat Commun; CHML promotes liver cancer metastasis by facilitating Rab14 recycle.; Authors - Tian-Wei Chen
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sophy (Verified Customer) (03-07-2023)

The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSCLC cells

FH

Malgorzata (Verified Customer) (08-17-2022)

The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: THP-1

RAB7A Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:55469-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

• Applications: Western Blot, Immunohistochemistry,
• Cell Tissue Type: HEKs

FH

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 Lysate

RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)

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