RAB9A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB9A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11420-1-AP

Synonyms

RAB9, Ras-related protein Rab-9A, Ras related protein Rab 9A

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Validation Data Gallery

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  WB analysis using 11420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11420-1-AP (RAB9A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11420-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11420-1-AP (RAB9A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11420-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11420-1-AP (RAB9A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11420-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11420-1-AP (RAB9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below

Product Information

11420-1-AP targets RAB9A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB9A fusion protein Ag1969
• Full Name: RAB9A, member RAS oncogene family
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC017265
• Gene Symbol: RAB9A
• Gene ID (NCBI): 9367
• RRID: AB_2175327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; DNA damage invokes mitophagy through a pathway involving Spata18.; Authors - Xiuli Dan
human	WB	Int J Mol Sci; The Amyotrophic Lateral Sclerosis M114T PFN1 Mutation Deregulates Alternative Autophagy Pathways and Mitochondrial Homeostasis.; Authors - Elisa Teyssou
human	WB	Virology; Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells.; Authors - Takeshi Yamasaki
mouse	IF	J Gen Virol; Characterization of intracellular localization of PrP(Sc) in prion-infected cells using a mAb that recognizes the region consisting of aa 119-127 of mouse PrP.; Authors - Yamasaki Takeshi T
human	WB	Technol Cancer Res Treat; Knockdown of Rab9 Suppresses the Progression of Gastric Cancer Through Regulation of Akt Signaling Pathway.; Authors - Yong Zhu ; KD Validated
	WB,IF	J Virol; Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport; Authors - Xiong-Nan Chen