Anticorps Polyclonal de lapin anti-RAB35

• Phare
• Validé par KD/KO

RAB35 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (3)

N° de cat : 11329-2-AP

Synonymes

GTP binding protein RAY, H ray, RAB1C, RAB35, Ras related protein Rab 1C, Ras related protein Rab 35, RAY

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Galerie de données de validation

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  WB analysis using 11329-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11329-2-AP

  WB result of RAB35 antibody (11329-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB35 transfected A431 cells.

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  WB analysis of human brain using 11329-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 11329-2-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 11329-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11329-2-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of A375 using 11329-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11329-2-AP

  IP result of anti-RAB35 (IP:11329-2-AP, 4ug; Detection:11329-2-AP 1:600) with mouse brain tissue lysate 2400 ug.

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  IHC staining of human breast cancer using 11329-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11329-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11329-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11329-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11329-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11329-2-AP (RAB35 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11329-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11329-2-AP (RAB35 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U-87 MG using 11329-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB35 antibody (11329-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A375, cellules HeLa, cellules MCF-7, cellules RAW 264.7, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MG U-87,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 36 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11329-2-AP cible RAB35 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB35 Protéine recombinante Ag1872
• Nom complet: RAB35, member RAS oncogene family
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC015931
• Symbole du gène: RAB35
• Identification du gène (NCBI): 11021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

Publications

Species	Application	Title
human	WB	Science; Identification of an oncogenic RAB protein.; Authors - Douglas B Wheeler ; KD Validated
human	WB	Cell Metab; Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.; Authors - Chaitali Mukherjee
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB	Nat Cell Biol; Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.; Authors - Alexander Wallroth
mouse	WB,IF	Sci Adv; LRRK2 mediates tubulation and vesicle sorting from lysosomes.; Authors - Luis Bonet-Ponce
human,mouse	WB,IHC,IF,CoIP	Nat Commun; A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.; Authors - Corallino Salvatore ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (03-15-2022)

A clean antibody to probe Rab35 with the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human postmortem brain lysate

RAB35 Antibody Western Blot validation (1:2000 dilution) in human postmortem brain lysate (Cat no:11329-2-AP)

FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RAB35 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11329-2-AP)

FH

Mayur (Verified Customer) (01-14-2019)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain tissue (Cortex)

RAB35 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain tissue (Cortex) (Cat no:11329-2-AP)

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