RAB11B-Specific Polyclonal antibody

RAB11B-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19742-1-AP

Synonyms

GTP binding protein YPT3, H YPT3, RAB11B, RAB11B-Specific, Ras related protein Rab 11B, YPT3

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Validation Data Gallery

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  WB analysis using 19742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 19742-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 19742-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19742-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19742-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 19742-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 19742-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19742-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19742-1-AP

  IP result of anti-RAB11B-Specific (IP:19742-1-AP, 4ug; Detection:19742-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

• 

  IHC staining of human lung using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, C6 cells, PC-12 cells, fetal human brain tissue, human testis tissue, mouse testis tissue, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung tissue, human brain tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

19742-1-AP targets RAB11B-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB11B, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_004218
• Gene Symbol: RAB11B
• Gene ID (NCBI): 9230
• RRID: AB_10642010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.

Publications

Species	Application	Title
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Tumour Biol; AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.; Authors - Zhaoyang Zeng
canine,rat	WB,IHC	J Histochem Cytochem; Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.; Authors - Hiroshi Gomi
human	WB,IF	J Neurosci; The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.; Authors - Wataru Otsu
human	WB,IP	Heliyon; Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9; Authors - Dong-Shan Yu