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RAB11A-Specific Polyclonal antibody
RAB11A-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 20229-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, human brain tissue, mouse colon tissue, SH-SY5Y cells, rat brain tissue, PC-12 cells, HeLa cells, MCF-7 cells, mouse lung tissue |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 11 publications below |
IHC | See 1 publications below |
IF | See 11 publications below |
Product Information
20229-1-AP targets RAB11A-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | RAB11A, member RAS oncogene family |
Calculated Molecular Weight | 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | NM_004663 |
Gene Symbol | RAB11A |
Gene ID (NCBI) | 8766 |
RRID | AB_10666202 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.
Protocols
Product Specific Protocols | |
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WB protocol for RAB11A-Specific antibody 20229-1-AP | Download protocol |
IHC protocol for RAB11A-Specific antibody 20229-1-AP | Download protocol |
IF protocol for RAB11A-Specific antibody 20229-1-AP | Download protocol |
IP protocol for RAB11A-Specific antibody 20229-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Thromb Haemost Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII. | ||
Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.
| ||
J Cell Biol BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia. | ||
EMBO Rep Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. | ||
Cell Mol Gastroenterol Hepatol Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis. | ||
Br J Pharmacol An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice. |