• Featured Product
  • KD/KO Validated

RAB11A-Specific Polyclonal antibody

RAB11A-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20229-1-AP

Synonyms

RAB11, Rab 11, RAB11A/B, RAB11A, Rab-11



Tested Applications

Positive WB detected inHEK-293T cells, human brain tissue, mouse colon tissue, SH-SY5Y cells, rat brain tissue, PC-12 cells, HeLa cells, MCF-7 cells, mouse lung tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20229-1-AP targets RAB11A-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB11A, member RAS oncogene family
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberNM_004663
Gene Symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_10666202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Protocols

Product Specific Protocols
WB protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
IHC protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
IF protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
IP protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Thromb Haemost

Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII.

Authors - Jenny Chia
humanWB

Proc Natl Acad Sci U S A

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Authors - Humbert Melissa C MC
  • KD Validated

J Cell Biol

BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.

Authors - William J Monis
mouseIF

EMBO Rep

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

Authors - Ye Chen
mouseWB

Cell Mol Gastroenterol Hepatol

Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis.

Authors - Olga A Mareninova
humanIF

Br J Pharmacol

An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.

Authors - Weisheng Lu