RAB11A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB11A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 20229-1-AP

Synonyms

RAB11A-Specific, RAB11, Rab 11, RAB11A/B, Rab-11

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Validation Data Gallery

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  WB analysis using 20229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20229-1-AP

  WB result of RAB11A-Specific antibody (20229-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A-Specific transfected HeLa cells.

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  WB analysis of rat brain using 20229-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 20229-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20229-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20229-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20229-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 20229-1-AP

  IP result of anti-RAB11A-Specific (IP:20229-1-AP, 3ug; Detection:20229-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HEK-293 using 20229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAB11A-Specific antibody (20229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, human brain tissue, mouse colon tissue, SH-SY5Y cells, rat brain tissue, PC-12 cells, HeLa cells, MCF-7 cells, mouse lung tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human small intestine tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 13 publications below

Product Information

20229-1-AP targets RAB11A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_004663
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• RRID: AB_10666202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Publications

Species	Application	Title
human	IF	J Thromb Haemost; Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII.; Authors - Jenny Chia
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC ; KD Validated
		J Cell Biol; BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.; Authors - William J Monis
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
mouse	WB	Cell Mol Gastroenterol Hepatol; Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis.; Authors - Olga A Mareninova
human	IF	Br J Pharmacol; An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.; Authors - Weisheng Lu