Anticorps Polyclonal de lapin anti-AMPK Alpha

• Phare
• Validé par KD/KO

AMPK Alpha Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

213

Avis (6)

N° de cat : 10929-2-AP

Synonymes

AMPK, AMPK alpha, AMPK alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

---

Galerie de données de validation

• 

  WB analysis using 10929-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10929-2-AP (AMPK Alpha 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10929-2-AP

  IP result of anti-AMPK Alpha (IP:10929-2-AP, 4ug; Detection:10929-2-AP 1:1000) with HeLa cells lysate 1600 ug.

• 

  IHC staining of human prostate cancer using 10929-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10929-2-AP (AMPK Alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10929-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10929-2-AP (AMPK Alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 205 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10929-2-AP cible AMPK Alpha dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poisson-zèbre, porc, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMPK Alpha Protéine recombinante Ag1367
• Nom complet: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC012622
• Symbole du gène: AMPK Alpha 1
• Identification du gène (NCBI): 5562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.10929-2-AP can recognize both AMPK Alpha 1 and AMPK Alpha 2.

Publications

Species	Application	Title
mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	WB,IP	Autophagy; The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.; Authors - Yongquan Hu ; KO Validated
	WB	Autophagy; A plant nonenveloped double-stranded RNA virus activates and co-opts BNIP3-mediated mitophagy to promote persistent infection in its insect vector.; Authors - Qifu Liang
human	WB	J Hazard Mater; Mitigation of honokiol on fluoride-induced mitochondrial oxidative stress, mitochondrial dysfunction, and cognitive deficits through activating AMPK/PGC-1α/Sirt3.; Authors - Dongmei Wang
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jing (Verified Customer) (01-27-2020)

tested in mouse heart lysate, with clear single band around 60 kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart lysate

FH

Jie (Verified Customer) (01-27-2020)

showed a single band at the correct size with mouse heart tissue section

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse heart tissue

FH

Yan (Verified Customer) (01-03-2020)

We tested this antibody in mouse heart tissue extract. It worked very well and showed a single band around 60kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

Produits associés

GTPBP3 Polyclonal antibody

10764-1-AP

FOXO3A Polyclonal antibody

10849-1-AP

CRTC2,TORC2 Polyclonal antibody

12497-1-AP

Tuberin/TSC2 Polyclonal antibody

24601-1-AP

Tuberin-Specific Polyclonal antibody

20004-1-AP