Anticorps Polyclonal de lapin anti-PHLPP

• Phare
• Validé par KD/KO

PHLPP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (1)

N° de cat : 22789-1-AP

Synonymes

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

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Galerie de données de validation

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  WB analysis of HeLa using 22789-1-AP

  WB result of PHLPP antibody (22789-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPP transfected HeLa cells.

• 

  WB analysis using 22789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22789-1-AP (PHLPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22789-1-AP

  IP result of anti-PHLPP (IP:22789-1-AP, 4ug; Detection:22789-1-AP 1:600) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human liver using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22789-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHLPP antibody (22789-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 29 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 5 publications below

Informations sur le produit

22789-1-AP cible PHLPP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHLPP Protéine recombinante Ag18794
• Nom complet: PH domain and leucine rich repeat protein phosphatase
• Masse moléculaire calculée: 1717 aa, 184 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC126277
• Symbole du gène: PHLPP
• Identification du gène (NCBI): 23239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.; Authors - Geethu Emily Thomas ; KD Validated
human,mouse	WB,IHC,IP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
mouse	WB	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
human	WB	EMBO Rep; USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.; Authors - Dana Goldbraikh ; KD Validated
mouse	WB	Cell Mol Immunol; CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription.; Authors - Yu Song
mouse	WB	Elife; PHLPP1 counter-regulates STAT1-mediated inflammatory signaling.; Authors - Ksenya Cohen Katsenelson ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!

• Applications: Western Blot
• Cell Tissue Type: SHED cell lines

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