Anticorps Monoclonal anti-PDGFR alpha

PDGFR alpha Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2C11B8

Avis (1)

N° de cat : 60045-1-Ig

Synonymes

CD140A, CD140a antigen, PDGF R alpha, PDGF receptor alpha, PDGFR2, PDGFRA, PDGFRα, PDGFR-α, Rhe PDGFRA

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Galerie de données de validation

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  WB analysis of THP-1 using 60045-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 60045-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 60045-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 60045-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of THP-1 using 60045-1-Ig

  1X10^6 THP-1 cells were stained with 0.20ug PDGFRA antibody (60045-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HT-1080, cellules HUVEC, cellules U-251
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

60045-1-Ig cible PDGFR alpha dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDGFR alpha Protéine recombinante Ag1385
• Nom complet: platelet-derived growth factor receptor, alpha polypeptide
• Masse moléculaire calculée: 123 kDa
• Poids moléculaire observé: 100-120 kDa, 140-160 kDa
• Numéro d’acquisition GenBank: BC015186
• Symbole du gène: PDGFRA
• Identification du gène (NCBI): 5156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition; Authors - Tiffany L. Dill
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.; Authors - Tiffany L Dill
mouse	WB	Metabolism; The vitamin D receptor regulates miR-140-5p and targets the MAPK pathway in bone development.; Authors - Wenting Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (11-25-2021)

HEK293 lysate - some non-specific bands below real band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

PDGFR alpha Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:60045-1-Ig)

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