Anticorps Polyclonal de lapin anti-CCM3/PDCD10

• Phare
• Validé par KD/KO

CCM3/PDCD10 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10294-2-AP

Synonymes

CCM3, CCM3/PDCD10, PDCD10, programmed cell death 10, TFAR15

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Galerie de données de validation

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  WB analysis using 10294-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10294-2-AP

  IP result of anti-CCM3/PDCD10 (IP:10294-2-AP, 4ug; Detection:10294-2-AP 1:500) with MCF-7 cells lysate 2120 ug.

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  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules PC-3, cellules Raji
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

10294-2-AP cible CCM3/PDCD10 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCM3/PDCD10 Protéine recombinante Ag0348
• Nom complet: programmed cell death 10
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC002506
• Symbole du gène: PDCD10
• Identification du gène (NCBI): 11235
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling.; Authors - Shan Wang ; KO Validated
human,mouse	WB,IHC	J Clin Invest; Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.; Authors - Chan Aubrey C AC ; KD Validated
mouse	WB	Development; Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration.; Authors - Angeliki Louvi ; KO Validated
mouse	WB	Aging (Albany NY); PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling; Authors - Jingdian Liu ; KO Validated
human,rat	WB,IF	J Cell Sci; CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.; Authors - Fidalgo Miguel M ; KD Validated
human	WB	Hum Mutat; A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion ​.; Authors - Grazia Nardella

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