MST4 Polyclonal antibody

• Featured Product
• KD/KO Validated

MST4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 10847-1-AP

Synonyms

STK26, MST3 and SOK1-related kinase, Mst3 and SOK1 related kinase, MST 4, MASK

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Validation Data Gallery

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  WB analysis using 10847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10847-1-AP (MST4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10847-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10847-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10847-1-AP

  IP result of anti-MST4 (IP:10847-1-AP, 4ug; Detection:10847-1-AP 1:500) with MCF-7 cells lysate 2400ug.

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  IHC staining of human liver using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10847-1-AP (MST4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10847-1-AP (MST4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 10847-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 10847-1-AP (MST4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, HL-60 cells, MCF-7 cells, L02 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

10847-1-AP targets MST4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MST4 fusion protein Ag1302
• Full Name: serine/threonine protein kinase MST4
• Calculated Molecular Weight: 16 kDa, 46 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC017213
• Gene Symbol: MST4
• Gene ID (NCBI): 51765
• RRID: AB_513907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.; Authors - Eric Peterman ; KD Validated
human	WB,IHC,IF	Int J Oncol; MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.; Authors - Zhen-Hai Lin ; KD Validated
mouse	WB,IF	Brain Res Bull; MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice.; Authors - Xiaodong Wu
human	IHC	Onco Targets Ther; MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression.; Authors - Hanxi Yu
mouse	WB,IF	Behav Neurol; MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.; Authors - Xiaodong Wu
human	WB	Antiviral Res; Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3; Authors - Tan Ming

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guillaume (Verified Customer) (07-21-2022)

Antibody worked well, band present at 55kDa

• Applications: Western Blot
• Cell Tissue Type: Mice liver tumor/ Mice healthy liver

MST4 Antibody Western Blot validation ( dilution) in Mice liver tumor/ Mice healthy liver (Cat no:10847-1-AP)