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- KD/KO Validated
PPP2CA Polyclonal antibody
PPP2CA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13482-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, human liver tissue, MCF-7 cells, HEK-293T cells |
Positive IP detected in | Hela cells, Jurkat cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 24 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Product Information
13482-1-AP targets PPP2CA in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PPP2CA fusion protein Ag4294 |
Full Name | protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform |
Calculated molecular weight | 309 aa, 36 kDa |
Observed molecular weight | 36 kDa |
GenBank accession number | BC031696 |
Gene symbol | PPP2CA |
Gene ID (NCBI) | 5515 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PPP2CA antibody 13482-1-AP | Download protocol |
IHC protocol for PPP2CA antibody 13482-1-AP | Download protocol |
IF protocol for PPP2CA antibody 13482-1-AP | Download protocol |
IP protocol for PPP2CA antibody 13482-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
Cell Res Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.
| ||
Acta Pharm Sin B WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit. | ||
Cardiovasc Res Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo. | ||
J Pineal Res Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload | ||
Redox Biol Sustained IKKβ phosphorylation and NF-κB activation by superoxide-induced peroxynitrite-mediated nitrotyrosine modification of B56γ3 and PP2A inactivation. |