PPP2CA Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP2CA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 13482-1-AP

Synonyms

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

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Validation Data Gallery

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  WB analysis of Jurkat using 13482-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13482-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13482-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 13482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Hela cells using 13482-1-AP

  IP result of anti-PPP2CA (13482-1-AP for IP and Detection) with HeLa cells.

• 

  IP experiment of Jurkat using 13482-1-AP

  IP Result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human liver using 13482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 13482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 13482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of MCF-7 using 13482-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: Jurkat cells, human liver tissue, MCF-7 cells, HEK-293T cells
• Positive IP detected in: Hela cells, Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

13482-1-AP targets PPP2CA in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2CA fusion protein Ag4294
• Full Name: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• Calculated molecular weight: 309 aa, 36 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC031696
• Gene symbol: PPP2CA
• Gene ID (NCBI): 5515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB,IP	Cell Res; Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.; Authors - Xiang Xu ; KD Validated
human	WB	Acta Pharm Sin B; WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.; Authors - Xiaomeng Gao
mouse	WB	Cardiovasc Res; Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.; Authors - Sarah-Lena Puhl
mouse	WB	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
human	IP	Redox Biol; Sustained IKKβ phosphorylation and NF-κB activation by superoxide-induced peroxynitrite-mediated nitrotyrosine modification of B56γ3 and PP2A inactivation.; Authors - Yi Hui Yee