Anticorps Polyclonal de lapin anti-PBXIP1

• Phare
• Validé par KD/KO

PBXIP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 12102-1-AP

Synonymes

HPIP, PBXIP1

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Galerie de données de validation

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  WB analysis of BxPC-3 using 12102-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12102-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12102-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12102-1-AP

  IP result of anti-PBXIP1 (IP:12102-1-AP, 3ug; Detection:12102-1-AP 1:800) with HeLa cells lysate 3000ug.

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  IHC staining of human lung cancer using 12102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12102-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12102-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules A549, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

12102-1-AP cible PBXIP1 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PBXIP1 Protéine recombinante Ag2746
• Nom complet: pre-B-cell leukemia homeobox interacting protein 1
• Masse moléculaire calculée: 731 aa, 81 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC016852
• Symbole du gène: PBXIP1
• Identification du gène (NCBI): 57326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).

Publications

Species	Application	Title
human	WB	J Clin Invest; Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.; Authors - Xu Xiaojie X ; KD Validated
human	WB,IF	Cell Death Differ; MDM2 restrains estrogen-mediated AKT activation by promoting TBK1-dependent HPIP degradation.; Authors - K Shostak
human	WB,IHC,IF	Oncogenesis; The interplay between HPIP and casein kinase 1α promotes renal cell carcinoma growth and metastasis via activation of mTOR pathway.; Authors - H Mai ; KD Validated
human	WB,IHC	Cancer Sci; Hematopoietic pre-B cell leukemia transcription factor interacting protein is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, migration, and invasion.; Authors - Yingying Feng ; KD Validated
human, mouse	WB, IHC	Sci Rep; HPIP is upregulated in colorectal cancer and regulates colorectal cancer cell proliferation, apoptosis and invasion.; Authors - Yingying Feng
human	IHC	IUBMB Life; HPIP is upregulated in liver cancer and promotes hepatoma cell proliferation via activation of G2/M transition.; Authors - Xu Xiaojie X ; KD Validated

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