PBX1 Polyclonal antibody

PBX1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 18204-1-AP

Synonyms

Homeobox protein PBX1, Homeobox protein PRL, PBX1, pre B cell leukemia homeobox 1, PRL

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Validation Data Gallery

• 

  WB analysis of A2780 using 18204-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18204-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18204-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18204-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A2780 using 18204-1-AP

  IP result of anti-PBX1 (IP:18204-1-AP, 4ug; Detection:18204-1-AP 1:500) with A2780 cells lysate 1000ug.

• 

  IF Staining of HeLa using 18204-1-AP

  Immunofluorescent analysis of HeLa cells using 18204-1-AP (PBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A2780 cells, HeLa cells
• Positive IP detected in: A2780 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 18204-1-AP is PBX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PBX1 fusion protein Ag12817
• Full Name: pre-B-cell leukemia homeobox 1
• Calculated Molecular Weight: 430 aa, 47 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC101578
• Gene Symbol: PBX1
• Gene ID (NCBI): 5087
• RRID: AB_10951860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b) and molecular weight of PBX1 is 47 kDa and 38 kDa.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human,rat	WB,IF	Int J Oral Sci; Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis; Authors - Xiao Yang
mouse	WB	J Neurochem; Decreased levels of constitutive proteasomes in experimental autoimmune encephalomyelitis may be caused by a combination of subunit displacement and reduced Nfe2l1 expression.; Authors - Kara L Shanley
mouse	WB	Neuroscience; Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion.; Authors - Zhengfang Lu
human	WB	Cell Death Dis; M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops; Authors - Hongda Lin
mouse	ChIP	Cell Mol Life Sci; Loss of PBX1 function in Leydig cells causes testicular dysgenesis and male sterility; Authors - Fei-Chen Wang