Anticorps Polyclonal de lapin anti-TrkB

TrkB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array

Conjugaison

Non conjugué

Publications

57

N° de cat : 13129-1-AP

Synonymes

GP145 TrkB, NTRK2, TrkB, TrkB tyrosine kinase

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Galerie de données de validation

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13129-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 13129-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13129-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spinal cord using 13129-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse cerebellum using 13129-1-AP

  IP result of anti-TrkB (IP:13129-1-AP, 4ug; Detection:13129-1-AP 1:300) with mouse cerebellum tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 13129-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 13129-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de porc, tissu de cervelet de souris, tissu de cervelet humain, tissu de moelle épinière de souris, tissu d'estomac humain
• Résultats positifs en IP: tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 53 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

13129-1-AP cible TrkB dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TrkB Protéine recombinante Ag3790
• Nom complet: neurotrophic tyrosine kinase, receptor, type 2
• Masse moléculaire calculée: 822 aa, 92 kDa
• Poids moléculaire observé: 90-95 kDa, 140-145 kDa
• Numéro d’acquisition GenBank: BC031835
• Symbole du gène: TrkB
• Identification du gène (NCBI): 4915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Publications

Species	Application	Title
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
rat	WB	Oxid Med Cell Longev; Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway.; Authors - Yaran Gao
mouse	WB	J Affect Disord; Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel; Authors - Hui Yao
rat	WB	ACS Chem Neurosci; Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.; Authors - Lian Liu
human	IHC	Am J Cancer Res; LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance.; Authors - Zhilan Li
human	WB,IF	Cell Biosci; A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.; Authors - Juan Yin

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