Neurotrophin 4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Neurotrophin 4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12297-1-AP

Synonyms

NTF4, NT-4, NT4, NT 4, Neutrophic factor 4

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Validation Data Gallery

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  WB analysis using 12297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12297-1-AP (Neurotrophin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 12297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using Neurotrophin 4 antibody (12297-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 12297-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 12297-1-AP (Neurotrophin 4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue
• Positive IF/ICC detected in: PC-3 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

12297-1-AP targets Neurotrophin 4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neurotrophin 4 fusion protein Ag2938
• Full Name: neurotrophin 4
• Calculated Molecular Weight: 210 aa, 22 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC012421
• Gene Symbol: Neurotrophin 4
• Gene ID (NCBI): 4909
• RRID: AB_10598309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	Int J Oncol; Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy.; Authors - Zhou Yang ; KD Validated
human	WB	Onco Targets Ther; Analysis of risk factors for colon cancer progression.; Authors - Zhou Yang