Anticorps Polyclonal de lapin anti-NQO1

• Phare
• Validé par KD/KO

NQO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

324

N° de cat : 11451-1-AP

Synonymes

DTD, DT diaphorase, DIA4, DIA 4, DHQU

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Galerie de données de validation

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  WB analysis using 11451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP (NQO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11451-1-AP

  WB result of NQO1 antibody (11451-1-AP, 1:1000) with si-control and si-NQO1 transfected HepG2 cells.

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  IP experiment of HepG2 using 11451-1-AP

  IP result of anti-NQO1 (IP:11451-1-AP, 4ug; Detection:11451-1-AP 1:300) with HepG2 cells lysate 3000 ug.

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  IHC staining of human colon cancer using 11451-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11451-1-AP (NQO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11451-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NQO1 antibody (11451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 11451-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human NQO1 (11451-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, cellules LNCaP, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 307 publications below
• IHC: See 24 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Informations sur le produit

11451-1-AP cible NQO1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Chèvre, Humain, Lapin, porc, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NQO1 Protéine recombinante Ag2009
• Nom complet: NAD(P)H dehydrogenase, quinone 1
• Masse moléculaire calculée: 274 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC007659
• Symbole du gène: NQO1
• Identification du gène (NCBI): 1728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

Publications

Species	Application	Title
human	WB	Nature; The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.; Authors - Kirill Bersuker ; KO Validated
mouse	WB	Sci Transl Med; Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease.; Authors - Yi Lu
human	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
mouse	WB	Nat Commun; Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.; Authors - Insup Choi
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang

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