Anticorps Polyclonal de lapin anti-NPHS2

NPHS2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications

48

N° de cat : 20384-1-AP

Synonymes

NPHS2, PDCN, Podocin, SRN1

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Galerie de données de validation

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  WB analysis of rat kidney using 20384-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20384-1-AP (NPHS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 20384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20384-1-AP (NPHS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of zebrafish embryos using 20384-1-AP

  The podocin antibody 20384-1-AP recognized the zebrafish pronephric glomeruli in zebrafish embryos (72 hour-old fish). IF result from Dr. Weibin Zhou.

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:600 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de rat,
• Résultats positifs en IHC: tissu rénal de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris, embryons de poisson-zèbre
• Résultats positifs en IF-Fro: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:300-1:1200
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 31 publications below
• IHC: See 7 publications below
• IF: See 25 publications below

Informations sur le produit

20384-1-AP cible NPHS2 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPHS2 Protéine recombinante Ag14213
• Nom complet: nephrosis 2, idiopathic, steroid-resistant (podocin)
• Masse moléculaire calculée: 383 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC029141
• Symbole du gène: NPHS2
• Identification du gène (NCBI): 7827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NPHS2 (also known as Podocin) is a membrane protein located on the podocyte foot process and is the critical component of the glomerular filtration barrier. Mutations of NPHS2 cause recessive steroidresistant nephrotic syndrome. Two isoforms of NPHS2 exist with molecular weights of 42 kDa and 35 kDa, respectively. (PMID: 21499232)

Publications

Species	Application	Title
human	WB,IF	Kidney Int; A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome; Authors - Valeryia Kuzmuk
mouse	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
rat	IF,WB	Front Immunol; Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy; Authors - Xin Lv
mouse	IF	Aging (Albany NY); MiR-320a induces diabetic nephropathy via inhibiting MafB.; Authors - Mengying He
	WB	Cell Physiol Biochem; Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage.; Authors - Zhi Zuo

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