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CD2AP Polyclonal antibody
CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 51046-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, mouse testis tissue, mouse brain tissue, Jurkat cells, A431 cells, HeLa cells |
Positive IP detected in | mouse brain tissue, HeLa cells |
Positive IF/ICC detected in | HepG2 cells, A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 5 publications below |
IF | See 3 publications below |
Product Information
51046-1-AP targets CD2AP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD2AP fusion protein Ag0622 |
Full Name | CD2-associated protein |
Calculated Molecular Weight | 637 aa, 70 kDa |
Observed Molecular Weight | 71 kDa |
GenBank Accession Number | BC157901 |
Gene Symbol | Cd2ap |
Gene ID (NCBI) | 12488 |
RRID | AB_670322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).
Protocols
Product Specific Protocols | |
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WB protocol for CD2AP antibody 51046-1-AP | Download protocol |
IF protocol for CD2AP antibody 51046-1-AP | Download protocol |
IP protocol for CD2AP antibody 51046-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Clin Invest Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function. | ||
Exp Ther Med Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation. | ||
J Biol Chem The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling
| ||
Int J Mol Sci The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model | ||
J Am Soc Nephrol Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes | ||
Int J Cardiol Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease
|