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  • KD/KO Validated

CD2AP Polyclonal antibody

CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 51046-1-AP

Synonyms

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein



Tested Applications

Positive WB detected inmouse kidney tissue, mouse testis tissue, mouse brain tissue, Jurkat cells, A431 cells, HeLa cells
Positive IP detected inmouse brain tissue, HeLa cells
Positive IF/ICC detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51046-1-AP targets CD2AP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD2AP fusion protein Ag0622
Full Name CD2-associated protein
Calculated Molecular Weight 637 aa, 70 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC157901
Gene Symbol Cd2ap
Gene ID (NCBI) 12488
RRIDAB_670322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Protocols

Product Specific Protocols
WB protocol for CD2AP antibody 51046-1-APDownload protocol
IF protocol for CD2AP antibody 51046-1-APDownload protocol
IP protocol for CD2AP antibody 51046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Clin Invest

Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.

Authors - Sei Yoshida
humanWB

Exp Ther Med

Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation.

Authors - Xintong Zheng
humanWB,IF

J Biol Chem

The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling

Authors - Tomohiko Iwano
  • KD Validated
mouseWB

Int J Mol Sci

The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model

Authors - Valentina Latina
mouseWB,IF

J Am Soc Nephrol

Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes

Authors - Naritoshi Shirata
humanWB

Int J Cardiol

Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease

Authors - Lili Yu
  • KD Validated