Anticorps Polyclonal de lapin anti-iNOS

• Phare
• Validé par KD/KO

iNOS Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

635

Avis (4)

N° de cat : 18985-1-AP

Synonymes

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

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Galerie de données de validation

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  WB analysis using 18985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18985-1-AP

  WB result of iNOS antibody (18985-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-iNOS transfected HeLa cells.

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  WB analysis of HeLa using 18985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 18985-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using iNOS antibody (18985-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18985-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18985-1-AP (iNOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 18985-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (18985-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 477 publications below
• IF: See 183 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below
• FC: See 2 publications below

Informations sur le produit

18985-1-AP cible iNOS dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nitric oxide synthase 2, inducible
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 110-130 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: NM_000625
• Symbole du gène: iNOS
• Identification du gène (NCBI): 4843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70 kDa band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publications

Species	Application	Title
mouse	WB	Adv Mater; Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis.; Authors - Yinan Li
mouse,rat	IF	Bioact Mater; Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments; Authors - Pengcheng Xiao
mouse	WB	Adv Sci (Weinh); 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure; Authors - Jiabin Zhang
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human,mouse	WB	J Exp Clin Cancer Res; Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling; Authors - Shengqi Wang
mouse	IF	Adv Healthc Mater; One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.; Authors - Shen Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yasuyo (Verified Customer) (03-01-2022)

This is one of the best IHC signaling I ever got for iNOS. Amazing image. I recommend this for iNOS detection by IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: skin

iNOS Antibody Immunohistochemistry validation (1:250 dilution) in skin (Cat no:18985-1-AP)

FH

Xie (Verified Customer) (10-06-2021)

Works nice for WB assay.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RAW 264.7

iNOS Antibody Western Blot validation (1:500 dilution) in RAW 264.7 (Cat no:18985-1-AP)

FH

Prasanth (Verified Customer) (08-19-2019)

This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Fibroblast Cells

FH

Elena (Verified Customer) (08-14-2019)

We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.

• Applications: Western Blot,

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