Anticorps Polyclonal de lapin anti-NCKAP1

NCKAP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 12140-1-AP

Synonymes

HEM2, KIAA0587, NAP 1, NAP1, NAP125, NCK associated protein 1, NCKAP1, p125Nap1

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Galerie de données de validation

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  WB analysis using 12140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12140-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse brain using 12140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cerveau de rat
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 3 publications below

Informations sur le produit

12140-1-AP cible NCKAP1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCKAP1 Protéine recombinante Ag2788
• Nom complet: NCK-associated protein 1
• Masse moléculaire calculée: 129 kDa
• Poids moléculaire observé: 115-125 kDa
• Numéro d’acquisition GenBank: BC015025
• Symbole du gène: NCKAP1
• Identification du gène (NCBI): 10787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

Publications

Species	Application	Title
human	WB	Cell Res; Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions.; Authors - Wu Shuang S
human,mouse	WB	Curr Biol; Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria.; Authors - Tak Shun Fung
mouse	WB	FASEB J; Tropomyosin-related kinase C (TrkC) enhances podocyte migration by ERK-mediated WAVE2 activation.; Authors - Sascha Gromnitza
		Biochem J; Selenoprotein S is Involved in Maintenance and Transport of Multiprotein Complexes.; Authors - Anton A Turanov
mouse	WB	Sci Rep; Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling.; Authors - Brent Wilkinson
human	WB	World J Gastroenterol; Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1.; Authors - Kun Li

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